AmScope FM320 TA Trinocular Compound Epi-Fluorescence Microscope, WF10x

The AmScope FM320TA epi-fluorescence trinocular compound microscope has a Siedentopf binocular mount with interchangeable pairs of 10x widefield and 16x widefield plan eyepieces, a reverse-mounted nosepiece with four DIN achromatic Brightfield objectives, four DIN fluorescence objectives, Brightfield and fluorescence illumination, a double-layer mechanical stage with a stage stop to protect slides and objectives from damage, and anti-mold surfaces to preserve optics in high-humidity areas. The trinocular head has a Siedentopf binocular mount with 55 to 75mm interpupillary adjustment, interchangeable pairs of WF10x and P16x eyepieces, and a fixed 45-degree vertical inclination to reduce eye and neck strain. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, eliminating the need to re-focus the image. The vertical trinocular mount accepts a camera with a C-mount or 23mm diameter. Dioptric adjustment accommodates individual eye-strength differences. The reverse-mounted revolving nosepiece accepts up to four 20mm objectives. A reverse-mounted nosepiece keeps objectives out of the way to prevent damage and contamination, and to enable more secure slide handling. Four DIN achromatic Brightfield objectives (4x, 10x, and 40xS, and 100xS) provide color correction of magnified images, and four DIN fluorescence plan objectives (4x, 10x, and 40xS, and 100xS) are designed for use with fluorescence illumination to provide improved focus over the entire viewing field. The 40xS objectives are spring loaded to prevent slide damage when focusing. The 100xS spring-loaded oil immersion objective uses oil between the specimen and the objective lens to provide increased resolution. Compound fluorescence microscopes are used in biological and metallurgical research and in industrial inspection applications to observe details illuminated by ultraviolet light.

The microscope has upper (incidental, episcopic) fluorescence illumination that reflects light off the specimen for enhanced visibility of opaque specimens, and lower (transmitted, diascopic) Brightfield illumination that transmits light up through the specimen for enhanced visibility of translucent and transparent objects. Brightfield (BF) illumination allows the specimen to absorb light, resulting in a dark image on a light background. Fluorescence illumination uses a mercury lamp to create ultraviolet light and excitation filters to filter unwanted wavelengths, allowing users to see details undetectable to other light sources. An epi-fluorescence microscope, or incident-light fluorescence, has the lamp and excitation filter mounted above the objective. Transmitted 20W halogen illumination provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. The 1.25 NA Abbe condenser is mounted on a rack-and-pinion control system, can be adjusted to control the distance of the light from the stage, has a swing-out color filter holder, and has an iris diaphragm to optimize the amount of light illuminating the specimen. The double-layer mechanical stage, with 1mm stage divisions, locks the slide into place and provides precise slide manipulation along the X- and Y-axis to allow coordinates to be recorded, enabling the viewer to return to a specific location on the slide. A stage stop prevents the stage from coming into contact with the slide and objectives. The stage is 5-1/2 x 6-5/16 inches (140 x 160mm) and has a traveling range of 2-3/4 x 2 inches (70 x 50mm). Graduated coaxial coarse and fine focus has a focusing range of 1-3/16" (30mm) and fine-focus divisions of 0.002mm (0.0000787") that enable measurements to be taken. Focus knob tension control prevents the stage from drifting out of focus. The cast-alloy enamel-coated body is durable and resistant to stains and corrosion.

SpecificationsMagnification range40x-1600xHeadCompound trinocularEyepieces (23mm)WF10x, P16xBrightfield objectives (20mm)4X, 10x, 40xS, 100xS (oil)Fluorescence objectives (20mm)4X, 10x, 40xS, 100xS (oil)StageDouble-layer mechanicalLighting configurationTransmitted (lower) and incidental (upper)Condenser1.25 NA AbbeDiaphragmIrisLight source20W Halogen with rheostat (transmitted), and mercury lamp (incidental)Illumination typeFluorescence and BrightfieldPower110V

Microscopes are instruments used to enhance the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, rather than looking through a lens. Microscopes can have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers to the size of an image. Resolution, also known as resolvant power, refers to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can control magnification through a fixed focus, or through a range of adjustments. They can also utilize LED, fluorescent, and mirror light sources to help control viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and in the medical, forensic science, and veterinary industries.

United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.

What's in the Box?

• AmScope FM320TA microscope with double-layer mechanical stage
• WF10x eyepieces, 23mm, one pair
• P16x eyepieces, 23mm, one pair
• 4x DIN achromatic objective, 20mm
• 10x DIN achromatic objective, 20mm
• 40xS DIN achromatic objective, 20mm
• 100xS (oil) DIN achromatic objective, 20mm
• 4x DIN fluorescence plan objective, 20mm
• 10x DIN fluorescence plan objective, 20mm
• 40xS DIN fluorescence plan objective, 20mm
• 100xS (oil) DIN fluorescence plan objective, 20mm
• Fluorescence head channel and filter selector
• Power box
• Mercury light housing box
• Fluorescence shield
• (2) Color filters; blue and green
• Spare halogen bulb
• Spare fuse
• Immersion oil, one bottle
• Instructions