15-PGDH Inhibitor Screening Kit (Fluorometric)

Important - Due to the nature of this product, all orders must be shipped to verifiable businesses at commercial addresses. K- Simple assay to screen/study/characterize COX- Inhibitors Simple method to screen 15-PGDH inhibitors High-throughput Compatible Includes 15-PGDH Inhibitor Control, 15-hydroxyprostaglandin dehydrogenase (15-PGDH, EC: 1.1.1.141) catalyzes the oxidation of Prostaglandins (PG) to 15-keto metabolites which show greatly reduced biological activity when compared to Prostaglandins. 15-PGDH catalyzes the rate-limiting step of PG catabolism. More recently, the inhibition of 15-PGDH has been reported as a negative regulator of tissue repair and inhibition of this enzyme can potentiate tissue regeneration in mouse s. Thus, pharmacological inhibition of 15-PGDH could have clinical implications by accelerating potential tissue regeneration after damage. In this assay, 15-PG is oxidized by 15-PDGH generating 15-Keto metabolites and oxidizing D. DH reduces a Fluorometric probe thus, generating an enhanced and stable fluorescence signal (Ex/Em=55/5 nm). In the presence of an inhibitor, the enzymatic activity is inhibited resulting No/Low fluorescence. BioVisionâ€s 15-PGDH Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of 15-PGDH inhibitors.